Flp-intm t-rextm 293 细胞系
Web293FTM细胞则是来源于Flp-InTMT-RETM293细胞,该细胞系中转染了ecotropic receptor质粒及MAPPIT(mammalian protein-protein interaction trap)报告质粒,该细胞系主要用于 … WebAug 5, 2024 · 293Flp-InTMT-RExTM Cells. 293Flp-In 293 T-REx细胞系是为了快速生成稳定的细胞系而设计的,它可以确保从Flp-In表达载体上获得的蛋白的均匀表达。这些细胞在转录活跃的基因组位点上包含一个稳定整合的FRT位点,有针对性的整合Flp-In表达载体,确保高水平目的基因。
Flp-intm t-rextm 293 细胞系
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WebThe Flp-In ™ T‑REx -293 cell line is shipped on dry ice. Store in liquid nitrogen vapor-phase upon receipt. The Flp-In™ T‑REx™-293 cell line is supplied as one vial containing 1 × … WebFeb 20, 2024 · 在我们以前的工作中,我们在四环素诱导型CMV启动子(HEK-293 Flp-In T-REx-Thermo)的控制下,建立了表达C末端3xFLAG标记的外来体成分EXOSC10(RRP6)的同基因HEK-293细胞系费雪科学)。. 该系统允许我们以与内源WT蛋白质相当的水平表达标记的EXOSC10蛋白质,并且使用磁性 ...
WebJul 2, 2012 · Als Kontrollen dienten mit dem Leervektor transfizierte und hCtr1 überexprimierende Flp-InTM T-RExTM-293-Zellen. Die rekombinanten hCtr2-Zellen erwiesen sich als signifikant weniger sensitiv gegenüber Cis- und Carboplatin als die Kontrollzellen. hCtr2 hatte hier also einen resistenzfördernden Effekt. Dies ist ein … WebFeb 13, 2024 · flp/frt 系统就属于位点特异性重组系统的一种。 FLP是一个重组酶(flippase,Flp),类似于Cre,它识别的位点是FRT,当FRT在同一条DNA上且方向相 …
WebThermo Fisher flp in t rex human embryonic kidney 293 hek293t cell line Flp In T Rex Human Embryonic Kidney 293 Hek293t Cell Line, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ... HEK293T-Flp-InTM T-RexTM (HEK293T) cells were maintained and cultured in DMEM media … WebApr 21, 2024 · Flp-In 293 T-REx细胞系是为了快速生成稳定的细胞系而设计的,它可以确保从Flp-In表达载体上获得的蛋白的均匀表达。 这些细胞在转录活跃的基因组位点上包含 …
WebOct 31, 2024 · HEK293细胞及其衍生株HEK293(human embryonic kidney 293),简称293细胞,来源于人胚胎肾细胞,由于在19号染色体整合了大约4.35 kb的腺病毒基因组片段,使其细胞周期以及细胞表型、核型等发生了 ... 293FTM细胞是由Flp-InTM T-RExTM 293细胞导入单嗜性(ecotropic)报告质粒和 ...
WebThe Flp-InTM T-RExTM 293 cells were generated by cotransfection of the constructed plasmid and pOG44 plasmid to express the target gene. Successfully recombined monoclonal cell lines were screened by hygromycin B resistance. Western blot and indirect immunofluorescence (IFA) were used to examine the expression of the target protein in ... michigan bigfoot conferenceWebFlp-In™ stable cell lines are isogenic and hence protein expression is constant across a population of cells. A useful addition to the Flp-In™ system (Flp-In™ T-Rex™) allows … michigan big ten champs sweatshirtsWebFigure 4-2. TUG-891 but not TUG-1197 displays low potency mediated activation of hFFA1. Flp-InTM T-RExTM 293 cells able to express hFFA1-mVenus-HA were seeded (75,000 cells /well) into poly-D-lysine coated black clear-bottom 96-well plates. Expression of hFFA1 was induced by the antibiotic doxycycline (100 ng/ml) for 18-24 hours. Cells were … the nordic cookbook magnus nilssonWebFigure 3-20. Relative expression of FLAG-hFFA4-mVenus and FLAG-hFFA4-GFP2 in a Flp-InTM T-RExTM 293 cell line. Flp-InTM T-RExTM 293 double stable cells able to express inducibly FLAG-hFFA4-mVenus and constitutively FLAG-hFFA4-GFP2 were seeded into poly-Dlysine coated black 96-well plates. Cells were treated with varying concentrations … michigan big ten championship towelWebFlp-In ™ 293 T-REx 细胞系设计用于快速生成稳定细胞系,确保通过 Flp-In™ 表达载体对您的目标蛋白质进行均一表达。这些细胞在转录活性基因组基因座处包含一个稳定整合的 … the nordic fiddlers blocWeb技术资料. 规格:. 1ml. Flp-In™系统使您可以稳定整合和表达感兴趣的基因,以提供单拷贝等基因细胞系。. Flp-In™表达会在您选择的哺乳动物细胞系基因组中引入一个Flp重组靶 … the nordic hotelWebSep 29, 2024 · 293FTM 细胞 是由 Flp-InTM T-RExTM 293 ... 总之, 293系列细胞经过长时间的改造与优化,已在多个领域实现了较多的应用,源井生物对于293与293T细胞也有丰富的基因编辑和细胞模型构建的经验,并拥有上千种293细胞的KO现货,覆盖超20种信号通 … michigan big ten championship 2021