WebJan 1, 1991 · The HPLC pattern of apo-lactoferrin heat-treated at pH 4.0 and 100°C was similar to that of unheated apo-lactoferrin. It resisted heating at pH 4.0 and 90°C for 5 min without any significant losses of iron-binding capacity, antigenic activity, or … WebLactoferrin is a protein found in many mammalian milks, especially in the first breast milks. In this application, a supplement containing lactoferrin was analyzed using a polymer-base reversed phase column, Asahipak C4P-50 4D. C4P-50 4D was proved to effectively detect lactoferrin in a supplement.
Journal of Dairy Research A rapid HPLC method for the …
WebThe present invention generally relates to enteric-coated particles containing lactoferrin. More specifically, the present invention provides an enteric-coated particle comprising (or consisting essentially of): a) a core comprising (or consisting essentially of) an inert core-forming material selected from cellulose polymer, sugar, sugar alcohol, starch and … WebDec 1, 2024 · The first RP-HPLC method was developed at Callaghan Innovation for determining bLF content and purity in a range of lactoferrin products (unpublished). The second RP-HPLC method used in this paper was a modified GB method, where the GB method was recently introduced by the Chinese Food and Drug Administration, China for … red lime broadstone
A RP-HPLC method for the detection and quantification of …
WebJan 1, 2004 · Abstract. Human lactoferrin was produced in genetically engineered rice. N -linked glycan structures of recombinant human lactoferrin were determined. The … WebJun 2, 2015 · Lactoferrin (LF) is a multifunctional whey protein with unique properties. An existing one step RP-HPLC method previously designed for the quantification of LF in whey, was now evaluated for... WebNov 1, 2006 · An RP-HPLC method was used to quantify β-lactoglobulin, α-lactalbumin and lactoferrin simultaneously. In a pH 3.0–5.0 lactoferrin-in-water suspension, at least 48% of undenatured lactoferrin remained after UHT. The highest level of undenatured lactoferrin was 81%, observed at pH 4.0; the iron binding capacity for this sample was 78%. richard hill c of e primary school