Phosphorimagery
WebSep 1, 1998 · Here we identify an immediate-early gene encoded by Kaposi’s sarcoma-associated herpesvirus (KSHV)/human herpesvirus eight (HHV8) that activates lytic cycle gene expression from the latent viral genome. The gene is a homologue of Rta, a transcriptional activator encoded by Epstein–Barr virus (EBV). KSHV/Rta activated KSHV … WebJul 23, 1998 · The SDS extract was immunoprecipitated with human, rabbit or rat sera as described 24 and monitored by SDS–PAGE and phosphorimagery. Calculation of the number of var mRNA species and var genes in...
Phosphorimagery
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WebThere is an urgent need to find new antibacterial agents to combat bacterial infections, including agents that inhibit novel, hitherto unexploited targets in bacterial cells. Amongst novel targets are two-component signal transduction systems (TCSs) which are the main mechanism by which bacteria sense and respond to environmental changes. TCSs … Webphosphorimagery and ImageQuant analysis. RNA was extracted from bulb, leaf and root samples of onions subjected to 32 long days, and from the equivalent short-day controls. A northern blot was produced and probed with $#P-labelled histone 2A cDNA, and the relative levels of ex-pression of the gene in each tissue were determined
WebNov 15, 1997 · Full-length cointegrate products linearized with Bam HI were purified by native gel electrophoresis and then resolved on a 0.6% alkaline agarose gel, which was dried for phosphorimagery. Linear scans of the radiolabeled products in each lane are shown. WebMay 15, 2001 · (A) Phosphorimagery after electrophoresis in denaturing 20% polyacrylamide gel with 5′-32 P-end-labeling of strand b of the four-way junction in Figure 1 after photo-irradiation. Lane 1, Maxam–Gilbert A+G; lane 2, C>T sequencing reactions (42); lane 3, photolysis at 313 nm for 10 min; lane 4 photolysis at 365 nm for 2 h followed by ...
WebAug 10, 1999 · When dry, turn off the vacuum and trap chiller and remove gel sandwich. Remove plastic wrap. Trim filter paper edges to close to gels. 7. Blank phosphor screen … Webphosphorimagery, followed by gel extraction using ddH 2O and filtration through micro-spin cellulose acetate membranes (Life Science Products, Inc.) (26). Purified products were …
WebJun 1, 2004 · Quantitative phosphorimagery revealed a low yield of cross-linked DNA for repandiol (~1%) relative to DEB (~16%) and DEO (~8%). The lower cross-linking efficiency of DEO relative to DEB has been noted previously [12] and attributed to the decrease in relative reactivity of diepoxyalkanes with increasing chain length [19].
WebSep 24, 2024 · In phosphor imaging, a storage phosphor screen captures the energy from the radioactive material. The storage screen contains a thin layer of crystals that absorb and store energy emitted by radioactive material. The storage screen is exposed to a … condos for rent in mokenaWebThis instrument has a range of 5 orders of magnitude and can detect radioactive signals using a phosphorimager screen with a 5- to 10-fold greater sensitivity than film-based … eddie murphy to be lovedWebAug 8, 2004 · The 3-nt abortive product was quantified by phosphorimagery, normalized, and the averages of three reactions (± 1 s.d.) were plotted. ( c) TFIIF can associate with the … eddie murphy the klumps with grandma runningPhotostimulated luminescence (PSL) is the release of stored energy within a phosphor by stimulation with visible light, to produce a luminescent signal. X-rays may induce such an energy storage. A plate based on this mechanism is called a photostimulable phosphor (PSP) plate and is one type of X-ray detector used in projectional radiography. Creating an image requires illuminating the plate twice: the first exposure, to the radiation of interest, "writes" the image, and a … eddie murphy the nutty professor 3WebAug 8, 2004 · Phosphorimagery scans showed that the RNase I treatment completely degraded the B2 RNA (data not shown). RNase I treatment did not affect the position at which preinitiation complexes migrated in ... condos for rent in mont tremblantWebSDS/PAGE and visualized by Phosphorimagery. Bands containing DNA cross-linked to Rpb1 or Rpb2 are indicated. 5570 www.pnas.org cgi doi 10.1073 pnas.0810738106 Yakovchuk … eddie murphy \u0026 paige butcherWebJun 29, 2001 · We demonstrate that the nonhydrolyzable ATP analog, ATPγS, supports the formation of an isolable leading strand complex that loads and replicates the lagging … eddie murphy till the money\\u0027s gone